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Katlyn K. Meier

Contact Information:
Mellon Institute 546
4400 Fifth Avenue
Pittsburgh, PA 15213
Lab: (412) 268-5059
Email: meierk@andrew.cmu.edu

Research Interests and Current Projects:
My research interests lie at the intersection between bioinorganic, biochemical and biomedical studies that incorporate Mössbauer and EPR spectroscopy. I am currently working on a variety of projects which utilize Mossbauer, EPR and Density Functional Theory calculations to help elucidate the chemical properties and structures of complexes that are important in oxygen activation. A few of my most recent projects (in collaboration with the Que and Lipscomb labs at the University of Minnesota) have focused on the bioinorganic spectroscopy of biomimetic peroxo intermediates, high-valence complexes, and the enzyme homoprotocatechuate 2,3 dioxygenase (HPCD).

Education:
Allegheny College, Meadville, PA
    • Bachelor of Science Degree in Physics
    • August 2005- May 2009
    • Minor: Philosophy
Carnegie Mellon University, Pittsburgh, PA
    • Fifth year graduate student
    • August 2010-present

Research Experience:
Allegheny College Physics Department, Meadville, PA                     

Fall 2008-Spring 2009

·         Collaborated with Dr. Doros Petasis in a two-semester long study using electron paramagnetic resonance spectroscopy and UV-Vis spectrophotometry to probe effects of temperature perturbations and solvent additions to spin crossover systems of Prussian Blue analogs with Fe-Ru cores.

·         Research was presented at the March 2009 APS Conference in Pittsburgh, PA.


University of Pittsburgh School of Medicine, MBSB department, Pittsburgh, PA
Summer 2008, Summer 2009

·         Assisted Dr. Rieko Ishima and post-doctoral fellow Joseph Walsh researching effects on protein structure and backbone dynamics in the presence and absence of alignment medium.

·         I was involved in all stages of this research including protein expression, purification, sample preparation, NMR data collection and NMR data analysis and interpretation.

·         Research was presented at the University of Pittsburgh Summer Undergraduate Research Conference and later published in Fall 2010.


Allegheny College Physics Department, Meadville, PA
Spring 2008

·         Assisted Professor Adele Poynor conducting a lab-based study using Surface Plasmon Resonance with the intention of creating and characterizing mixed hydrophobic and hydrophilic surface layers.

·         This research was displayed in poster format in the Allegheny College Physics Department for the 2008-2009 terms.



Publications:

·         Liu, J.; Meier, K.; Tian, S.; Zhang, J.; Guo, H.; Schulz, C. E.; Robinson, H.; Nilges, M. J.; Munck, E.; Lu, Y. “Redesigning the Blue Copper Azurin into a Redox-Active Mononuclear Nonheme Iron Protein: Preparation and Study of Fe(II)-M121E Azurin.” J. Am. Chem. Soc. 2014, 136, pp 12337-12344.

 

·         Chiang, C.-W.; Kleespies, S. T.; Stout, H. D.; Meier, K. K.; Li, P.-Y.; Bominaar, E. L.; Que, Jr., L.; Munck, E.; Lee, W.-Z. “Characterization of a Paramagnetic Mononuclear Nonheme Iron-Superoxo Complex.” J. Am. Chem. Soc. 2014, 136, pp 10846-10849.

 

·         England, J.; Bigelow, J. O.; Van Heuvelen, K. M.; Farquhar, E. R.; Martinho, M.; Meier, K. K.; Frisch, J. R.; Munck, E.; Que, Jr., L. “An Ultra-Stable Oxoiron(IV) Complex and Its Blue Conjugate Base.” Chem. Sci. 2014, 5, pp 1204-1215.

 

·         Oloo, Williamson N.; Meier, Katlyn K., Wang, Y.; Shaik, S.; Munck, E., Que Jr., Lawrence. “Identification of a low-spin acylperoxoiron(III) intermediate in bio-inspired non-heme iron-catalysed oxidations.” Nature Commun. 2013, 5, pp 3046-3054.

 

·         Li, Feifei; Van Heuvelen, Katherine M.; Meier, Katlyn K.; England, Jason; Münck, Eckard; Que, Jr., Lawrence.  “Sc3+ triggered oxoiron(IV) formation from O2 and its nonheme iron(II) precursor via a Sc3+-peroxo-Fe3+ intermediate.” J. Am. Chem. Soc. 2013, 135 (28), pp 10198-10201.

 

·         Banerjee, Rahul; Meier, Katlyn K.; Münck, Eckard; Lipscomb, John D. “Intermediate P* from Soluble Methane Monooxygenase Contains a Diferrous Cluster.” Biochemistry 2013, 52 (25), pp 4331-4342.

 

·         Mbughuni, Michael M.; Meier, Katlyn K.; Münck, Eckard.; Lipscomb, John D.  “Substrate-Mediated Oxygen Activation by Homoprotocatechuate 2,3-Dioxygenase: Intermediates Formed by a Tyrosine 257 Variant.”  Biochemistry 2012, 51 (44), pp 8743-8754.

 

·         Cranswick, Matthew A.; Meier, Katlyn K.; Shan, Xiaopeng; Stubna, Audria; Kaizer, Joszef; Mehn, Mark P.; Münck, Eckard; Que, Jr., Lawrence.  “Protonation of a Peroxodiiron(III) Complex and Conversion to a Diiron (III/IV) Intermediate: Implications to Proton-assisted O-O Bond Cleavage in Nonheme Diiron Enzymes.”  Inorganic Chemistry 2012, 51 (19), pp 10417-10426.

 

·         Van Heuvelen, Katherine M.; Fiedler, Adam T.; DeHont, Raymond; Shan, Xiaopeng; Meier, Katlyn K.; Bominaar, Emile; Münck, Eckard; Que, Jr., Lawrence.   “One-electron oxidation of an oxoiron(IV) complex to form an  [O=FeV=NR]+ center.” PNAS 2012, 109 (30), pp 11933-11938.

 

·         Mbughuni, Michael M.; Chakrabarti, Mrinmoy; Hayden, Joshua A.; Meier, Katlyn K.; Dalluge, Joseph J.; Hendrich, Michael P.; Münck, Eckard; Lipscomb, John D.  “Oxy-intermediates of homoprotocatechuate 2,3-dioxygenase: facile electron transfer between substrates.”  Biochemistry 2011, 50, pp 10262-10274.

 

·         Li, Feifei; Meier, Katlyn K.; Cranswick, Matthew A.; Chakrabarti, Mrinmoy; Van Heuvelen, Katherine M.; Münck, Eckard; Que, Jr., Lawrence. “Characterization of a High-Spin Non-Heme FeIII-OOH Intermediate and Its Quantitative Conversion to an FeIV=O Complex.” J. Am. Chem. Soc. 2011, 133 (19), pp 7256-7259.

 

·         Walsh, Joseph D.; Meier, Katlyn; Ishima, Rieko; Gronenborn, Angela M.  “NMR Studies on Domain Diffusion and Alignment in Modular GB1 Repeats.” Biophysical Journal 2010, 99, pp 2636-2646.

 

 

Invited Talks:

·         Meier, Katlyn K. (6 December 2013) “Studies of an Fe(II) dioxygenase and its intermediates using Mössbauer Spectroscopy and DFT.” Research was presented in a 20 minute lecture as part of the 9th Annual Mini-Symposium on Metals in Biological Systems held at Duquesne University, Pittsburgh, PA.

 

·         Meier, Katlyn K. (20 June 2013) “At the Frontier of Oxygen Activation Chemistry: The hunt for FeV=O Centers.” Research was presented in a 40 minute lecture as part of the Chemistry Graduate Student Seminar Series at Carnegie Mellon University, Pittsburgh, PA.

 

·         Meier, Katlyn K. (2 November 2012) “The New Frontier: Novel FeV=O Centers.” Research was presented in a 60 minute lecture as part of the joint Physics/Chemistry seminar series at Allegheny College, Meadville, PA. In an additional 20-minute session, I presented an overview of some of the research being conducted in labs at CMU.

 

·         Meier, Katlyn K.; Van Heuvelen, K.M.; Fiedler, A.T.; DeHont, R.; Shan, X.; Bominaar, E.; Münck, E.; Que, Jr., L.  (7 June 2012) “Spectroscopic Characterization of Novel FeV=O Centers.”  Research was presented at the Pennsylvania State University Bioinorganic Training Workshop as a 75-minute talk including real-time tutorials for an audience of 60+ graduate students and post-docs and 10+ faculty, State College, PA.

 

·         Meier, Katlyn K.; Van Heuvelen, K.M.; Fiedler, A.T.; DeHont, R.; Shan, X.; Bominaar, E.; Münck, E.; Que, Jr., L.  (27 January 2012) “One-electron Oxidation of an Oxoiron(IV) Complex.”  Research was presented at the Gordon Research Seminar as a 30-minute talk, Ventura, CA.

 

·         Meier, Katlyn K. (6 October 2011) "Enhancing the Information Content of EPR Using High-Frequency Techniques." Presented in the Graduate Seminar series as a 45-minute talk, Carnegie Mellon University, Pittsburgh, PA.

 

·         Meier, Katlyn K.; Walsh, J.; Ishima, R. (1 August 2008) “Determination of Backbone Dynamics in Single and Dual Domain Protein GB1: Comparison in the Presence and Absence of Alignment Media.” Research was presented as a 15-minute talk at the University of Pittsburgh Summer Undergraduate Research Conference held in Pittsburgh, PA.

 

 

Presentations:

·         Meier, K. K.; Rogers, M.; Kovaleva, E. G.; Bominaar, E. L.; Munck, E.; Lipscomb, J. D. (10 August 2014) “Characterization of a new, long-lived intermediate in H200C homoprotocatechuate 2,3 dioxygenase by Mossbauer, EPR, and DFT methods.” 248th ACS National Meeting and Exposition – Inorganic Division, San Francisco, CA.

 

·         Meier, Katlyn K.; Mbughuni, Michael M.; Munck, Eckard; Lipscomb, John D.  (22 July 2013-27 July 2013) “Probing the Reaction Cycle of Y257F Homoprotocatechuate 2, 3 Dioxygenase: Mössbauer and Density Functional Theory characterization of two short-lived intermediates.” Poster was presented during two scheduled poster sessions at the 16th International Conference on BioInorganic Chemistry held in Grenoble, France.

 

·         Meier, Katlyn K.; Van Heuvelen, K.M.; Fiedler, A.T.; DeHont, R.; Shan, X.; Bominaar, E.; Münck, E.; Que, Jr., L.  (31 May 2012-9 June 2012) “Spectroscopic Characterization of Novel FeV=O Centers.”  Poster was presented at the Pennsylvania State University Bioinorganic Training Workshop, State College, PA.

 

·         Meier, Katlyn K.; Li, Feifei; Cranswick, Matthew A.; Chakrabarti, Mrinmoy; Van Heuvelen, Katherine M.; Münck, Eckard; Que, Jr., Lawrence. (5 April 2012) “Characterization of a High-Spin Nonheme FeIII–OOH Intermediate and Its Conversion to an FeIV=O Complex.” Poster presented at the annual Innovation with Impact Research Exhibition during Graduate Student Appreciation Week, Carnegie Mellon University, Pittsburgh, PA.

 

·         Meier, Katlyn K.; Van Heuvelen, K.M.; Fiedler, A.T.; DeHont, R.; Shan, X.; Bominaar, E.; Münck, E.; Que, Jr., L.  (25-28 January 2012) “One-electron Oxidation of an Oxoiron(IV) Complex.”  Poster was presented at the Gordon Research Seminar, Ventura, CA.

 

·         Meier, Katlyn K.; Li, Feifei; Cranswick, Matthew A.; Chakrabarti, Mrinmoy; Van Heuvelen, Katherine M.; Münck, Eckard; Que, Jr., Lawrence. (10 August 2011) “Characterization of a High-Spin Nonheme FeIII–OOH Intermediate and Its Conversion to an FeIV=O Complex.” Poster presented during the poster session at the International Conference on Biological Inorganic Chemistry, Vancouver, BC Canada.

 

·         Meier, Katlyn K.; Nocera, T.; Abood, R.; Chen, M.; Hilfiger, M.; Petasis, D.; Achim, C.; Dunbar, K. (16-20 March 2009) “Electron Paramagnetic Resonance Spectroscopic Studies of Cyanide-Bridged Fe/Os and Fe/Ru Clusters.” Poster presented at the 2009 APS March Meeting, Pittsburgh, PA.

 

·         Meier, Katlyn K.; Walsh, J.; Ishima, R. (17 October 2008) “Determination of Backbone Dynamics in Single and Dual Domain Protein GB1: Comparison in the Presence and Absence of Alignment Media.” Research was presented at the poster session of the Allegheny College Inaugural Symposium in Meadville, PA.

 

 

updated 12/2014