Protein Identification
by Mass Spectrometry
at the Center for Molecular Analysis
Sample rates: Carnegie
Mellon Non-CMU
Peptide map (a
fingerprint of tryptic peptides): $35.00/sample $50.00/sample
The protein is purified and digested by the sample
submission lab. We may include a sample
clean up step before MS analysis. We provide the peptide MALDI-TOF mass
spectrum.
Submit a minimum of 5
pmoles.
Protein digestion chemistry and MS: $200.00/sample $250.00/sample
Protein is purified by sample submission lab. We do the reduction, alkylation and digestion
steps. We may include a sample clean up
step before MS analysis. We provide a peptide
MALDI-TOF mass spectrum to submitter.
Submit a
minimum of 5 pmoles.
Please
inquire if the experiments listed above do not identify your protein. A lack of identification may be due to
discovery of an unknown protein, impure protein or insufficient sample
quantities.
Advanced protein identification is done on a collaborative basis at
$15.00/hour of mass spectrometry
time. We provide complete interpretation of the
mass spectrum. We also includ
advanced purification techniques, advanced data base searching and peptide de
novo sequencing as required. Our
laboratory is capable of analyzing protein samples <1 pmole using our advanced
techniques.