Carnegie Mellon




3-D Structure of Human Serum Albumin

CMA Homepage
Information for users
Mass Spectrometry
Photon Spectroscopy
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Mark E. Bier
Director, CMA
412-268-3540 (office)
412-268-6897 (fax)

Roberto R. Gil

Director, NMR Facilities


Carnegie Mellon University
Department of Chemistry
Mellon Institute Bldg.
4400 Fifth Avenue
Pittsburgh, PA 15213-2683


Photon Spectroscopy

FTIR-NIR Spectrometer, MI 551

(Mattson ATI Affinity 60AR)


         Spectral range: MIR 4000 to 400-cm-1, NIR 10000 to 2000-cm-1

         Fiber optics option: Remspec Mid-IR fiber optic immersion probe system for reaction monitoring. This IR probe has a spectral range of 5000-cm-1 to 900cm1

User Requirements:

         You must supply a charge number.

         You must supply your own windows. (KBr, NaCl...etc)

         You must take a short tutorial course provided in the manual before using instrument.



UV-VIS-NIR Spectrometer, MI 551

(Perkin-Elmer Lambda 900)

PE Lambda 900 USER'S MANUAL in PDF format. 


         Spectral range:

o        3300-nm to 185-nm ( to 175-nm with nitrogen purge)

         Wavelength accuracy:

o        0.08-nm UV-VIS

o        0.32-nm NIR

         Wavelength reproducibility:

o        std. dev. = 0.008 for n=10 UV-VIS range

o        std. dev. = 0.04 for n=10 NIR range

         Spectral bandwidth:

o        0.05-nm to 5.00-nm in 0.01-nm increments UV-VIS range

o        0.2-nm to 20-nm in 0.04-nm increments NIR range

         Photometric accuracy:

o        Absorbance 0.003 at Abs. = 1

o        Absorbance 0.002 at Abs. = 0.5

         Two Fiber optic probe options:

o        Hellma standard fiber optic immersion probe

         300nm to 1100nm UV-VIS with cables

         400nm to 2300nm NIR with cables

         150 °C max temperature

         probe diameter 20mm

o        Hellma fiber optic all-quartz immersion probe

         400nm to 2300 nm NIR

         10mm light path

         15mm diameter, quartz measuring head

         18mm diameter, quartz tube

         150 °C maximum temperature

         -180 °C minimum temperature

         probe length 265mm

         good for corrosive mixtures



CD, MI 551

(JASCO 715)

CD spectropolarimetry utilizes left- and right- circularly polarized beams of light to excite optically active samples. The difference in emission intensities released perpendicular to the excitation light is then measured for the sample, and the temperature scan data and spectrum are obtained and displayed.

Parameters and Options:

         Spectral range: 165-900nm.

         Temperature range: -10 to 110 Celsius

         Spatial band width: .1, .2, .5, 1, 2, 5, 10nm, manual

         Slit width: 1-300 m

         Measurement sensitivity: 1, 2, 5, 10, 20, 50, 100, 200, 500, 100mdeg/FS

         Magnetic Adjustments

         2D or 3D spectrum

         Continuous or step spectrum


MI 551

MI=Mellon Institute Building

Status: See reservation calendar