Carnegie Mellon

  

 

 


3-D Structure of Human Serum Albumin

CMA Homepage
Information for users
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NMR
Photon Spectroscopy
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Mark E. Bier
Director, CMA
mbier@andrew.cmu.edu
412-268-3540 (office)
412-268-6897 (fax)


Roberto R. Gil

Director, NMR Facilities

rgil@andrew.cmu.edu

412-268-4313(office)


Carnegie Mellon University
Department of Chemistry
Mellon Institute Bldg.
4400 Fifth Avenue
Pittsburgh, PA 15213-2683

 



Photon Spectroscopy


FTIR-NIR Spectrometer, MI 551

(Mattson ATI Affinity 60AR)

Capabilities:

·         Spectral range: MIR 4000 to 400-cm-1, NIR 10000 to 2000-cm-1

·         Fiber optics option: Remspec Mid-IR fiber optic immersion probe system for reaction monitoring. This IR probe has a spectral range of 5000-cm-1 to 900cm1

User Requirements:

·         You must supply a charge number.

·         You must supply your own windows. (KBr, NaCl...etc)

·         You must take a short tutorial course provided in the manual before using instrument.

 

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UV-VIS-NIR Spectrometer, MI 551

(Perkin-Elmer Lambda 900)

PE Lambda 900 USER'S MANUAL in PDF format. 

Capabilities:

·         Spectral range:

o        3300-nm  to 185-nm ( to 175-nm with nitrogen purge)

·         Wavelength accuracy:

o        ± 0.08-nm UV-VIS

o        ± 0.32-nm NIR

·         Wavelength reproducibility:

o        std. dev. = 0.008 for n=10 UV-VIS range

o        std. dev. = 0.04 for n=10 NIR range

·         Spectral bandwidth:

o        0.05-nm to 5.00-nm in 0.01-nm increments UV-VIS range

o        0.2-nm to 20-nm in 0.04-nm increments NIR range

·         Photometric accuracy:

o        Absorbance ± 0.003 at Abs. = 1

o        Absorbance ± 0.002 at Abs. = 0.5

·         Two Fiber optic probe options:

o        Hellma standard fiber optic immersion probe

°         300nm to 1100nm UV-VIS with cables

°         400nm to 2300nm NIR with cables

°         150 °C max temperature

°         probe diameter 20mm

o        Hellma fiber optic all-quartz immersion probe

°         400nm to 2300 nm NIR

°         10mm light path

°         15mm diameter, quartz measuring head

°         18mm diameter, quartz tube

°         150 °C maximum temperature

°         -180 °C minimum temperature

°         probe length 265mm

°         good for corrosive mixtures

 

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CD, MI 551

(JASCO 715)

CD spectropolarimetry utilizes left- and right- circularly polarized beams of light to excite optically active samples. The difference in emission intensities released perpendicular to the excitation light is then measured for the sample, and the temperature scan data and spectrum are obtained and displayed.

Parameters and Options:

·         Spectral range: 165-900nm.

·         Temperature range: -10 to 110 Celsius

·         Spatial band width: .1, .2, .5, 1, 2, 5, 10nm, manual

·         Slit width: 1-300 µm

·         Measurement sensitivity: 1, 2, 5, 10, 20, 50, 100, 200, 500, 100mdeg/FS

·         Magnetic Adjustments

·         2D or 3D spectrum

·         Continuous or step spectrum

 

MI 551

MI=Mellon Institute Building

Status: See reservation calendar