CMA Homepage  Information for users  Reservations  Mass Spectrometry  NMR  Photon Spectroscopy Current Rates  Sample Drop-off  Useful Links  Mark E. Bier Director, CMA mbier@andrew.cmu.edu  412-268-3540 (office)  412-268-6897 (fax)  Roberto R. Gil Director, NMR Facilities rgil@andrew.cmu.edu 412-268-4313(office) Carnegie Mellon University  Department of Chemistry  Mellon Institute Bldg. 4400 Fifth Avenue Pittsburgh, PA 15213-2683	NMR Facility The NMR facility is located in Room 302 of the Mellon Institute. We have two Bruker NMR spectrometers: the 300 and 500 MHz Avance systems, installed in summer of 2003. We can perform Multinuclear and Multidimensional NMR Experiments in both Direct and Reverse Detection Mode using Gradient-Assisted Spectroscopy (GRASP). (Funded in part by NSF grant # CHE-9808188) Bruker Avance™ 500 500 MHz (^1H Frequency) NMR Instrument Specifications: · Actively Shielded Superconducting Magnet with 5.4 cm bore, operation field at 11.744 Tesla. Bruker Orthogonal Shim System (BOSS ™-2) with 28 shim gradients   · Two RF Channels   · Variable Temperature Control Unit with Low Temperature accessory (Nitrogen Evaporator)   · Equipped with Z-only Gradient Assisted Spectroscopy (GRASP ™ II) accessory for the generation of gradients with up to 50 Gauss/cm   · Multinuclear Inverse Broadband (BBI) probe (5mm sample diameter), ^1H observe with broadband decoupling coil tunable from ^109Ag (23.27 MHz) to ^31P (202.45 MHz)   Windows 2000 Workstation: Bruker NMR Suite Software package for acquisition of 1D, 2D and 3D NMR data. The suite includes: XWINNMR 3.5, XWINPLOT, ICON-NMR, NMR-CHECK, and NMR-SIM  Bruker Avance™ 300 300 MHz (^1H Frequency) NMR Instrument Specifications: · Superconducting Magnet with 5.4 cm bore, operation field at 7.049 Tesla. Bruker Orthogonal Shim System (BOSS ™-1) with 20 shim gradients   · Two RF Channels   · Equipped with Z-only Gradient Assisted Spectroscopy (GRASP ™ II) accessory for the generation of gradients with up to 50 Gauss/cm   · Variable Temperature Control Unit with Low Temperature accessory (Nitrogen Evaporator)   · Multinuclear Broadband Observe (BBO) probe (5mm sample diameter) to directly observe nuclei covering the range from ^109Ag (13.97 MHz) to ^31P (121.49 MHz), with 1H decoupling.   Windows 2000 Workstation: Bruker NMR Suite Software package for acquisition of 1D, 2D and 3D NMR data. The suite includes: XWINNMR 3.5, XWINPLOT, ICON-NMR, NMR-CHECK, and NMR-SIM  Capabilities: Our Facility is capable of performing a wide variety of NMR experiment. Some of the most common experiments are listed below by category. Routine NMR Spectroscopy Standard ^1H, ^13C, ^31P, ^19F, ^15N, ^29Si NMR experiments Decoupling Techniques Homonuclear Decoupling 1D Nuclear Overhauser Difference Spectroscopy Dynamic NMR Spectroscopy High and Low Temperature Experiments Saturation Transfer Experiments EXSY (EXchange SpectroscopY) 1D Multipulse Sequences Measurement of the Spin-Lattice Relaxation Time T1 Measurement of the Spin-Spin Relaxation Time T2 ^13C NMR APT (Attached Proton Test) ^13C DEPT- 45, 90 and135 Water Suppression by Presaturation Water Suppression by the Jump and Return Method Multinuclear Multidimensional NMR Spectroscopy 2D Homonuclear Correlation Experiments: COSY-Experiment and its variants, NOESY, ROESY, TOCSY. 2D Heteronuclear Correlation Experiments: HETCOR, COLOC, HMQC, HSQC, HSQC-DEPT, HMBC. NMR Spectroscopy with Pulsed Field Gradients Measurement of Diffusion Constant using the Pulsed Gradient Spin-Echo Experiment WATERGATE (water suppression experiment) Water Suppression by Excitation Sculpting Plus all the Gradient-Selected Multidimensional Experiments like gs-COSY, gs-TOCSY, gs-NOESY, gs-HSQC, gs-HMBC, etc.  For training, please contact Roberto Gil (rgil@andrew.cmu.edu), MI Room 302, telephone: x8-4313